CSF-1, IL-34 and calprotectin levels were analysed by ELISA according to instructions provided by the manufacturer (R&D Systems, Minneapolis, Minnesota, USA). 0200-0729. Versteegh, Yoran H. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that. TDP-43 stress granular cells were treated with 70% ice-cold. Absorbance was read at 405 nm on a microplate reader (SPECTRAmax 340 PC). 0200-7027. Molecular Devices Spectramax 340PC instructions. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax. 2. Developed plates were read on MSD reader Sector Imager S 600, Model 1200, with Discovery Workbench Software (version 4. Amino Acid Analysis. 94 g calcium chloride dihydrate (both. 3. using SpectraMax i3 (Molecular devices, San Jose, USA). The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. The results are expressed as the percentage inhibition of BrdU incorporation compared to the untreated control. Application note showing how SpectraMax® i3x and SpectraMax®iD5 Multi-Mode Microplate Readers are used to performGq-coupled receptor assays using HTRF technology. Awareness Technology Inc. Fully tested with WarrantyThe SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). All absorbance measurements were taken on a SpectraMax 340 PC reader equipped with SoFTMax Pro software. SpectraMax QuickDrop Micro-Volume Spectrophotometer. The membranes were. Spectrophotometer (SpectraMax 340). Oxidative stress induction by H 2 O 2 and intracellular ROS analysis in L-929 cells. 1 0 100 80 60 40 20 0 4. Money Saved: $340. This Molecular Devices SPECTRAmax 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. This was followed by an additional incubation at 37 °C for 30–60 min. 190-1000 nm, tunable in 1 nm increments. Time-resolved fluorescence was measured on the SpectraMax i3x and SpectraMax iD5 readers using preconfigured protocols in SoftMax® Pro Software (see Tables 2 and 3 for instrument-specific settings). The slope of the linear portion of the kinetic curve generated from each well was used to. Call 1-800-745-2710. PerkinElmer offers a variety of Microplates For Absorbance and Colorimetric Assays. A standard curve was constructed by sequential dilution of a TNFα standard from 2000-15 pg/mL. Details. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deep UV spectra must be used. When you do a read at wavelengths below 340 nm, you must use special UV-transparent, disposable or quartz plates to allow transmission of the deep UV spectra. Awareness Technology, Inc. If depot service is the best option for you we will assist you with logistics to help you keep. 5. Example: [Drawer]. The Molecular Devices SpectraMAX absorbance microplate readers mimic a dual beam spectrophotometer with a patented multichannel optical design. Measurements of OD at 540 nm were then performed in a microtiter plate reader (SpectraMax 340 microplate reader; Molecular Devices Corp. 5. The narrow bandwidth used by the model increases measurement accuracy and broad analysis over. The activity of glutathione peroxidase (GPX) was quantified spectrophotometrically at 340 nm, following the method of Takeda et al. spectramax i3 multi-mode platform, spectramax i3, spectramax i3 system, scanlater western blot system, spectramax minimax imaging cytometer, softmax pro software, metamorph software, spectramax i3 platform. SpectraMax® Microplate Readers and SoftMax® Pro Software. 05% at 340 nm Photometric stabilization Instantaneous Photometric drift None—continuous referencing of monochromatic output Calibration Automatic before first kinetic read and. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. Plate reader (SpectraMax 340 PC plate reader or comparable plate reader). 10× trypsin 0. ) at 490 nm. Like CD3, the CD95 death receptor requires binding of immobilized or multimeric antibodies to be activated effectively. 2 M DEEMM were mixed in a microtube,. The maximum specific growth rate μ was calculated from the exponential growth phase ( 52 ) and plotted against the concentration of the different drugs. Oxidative stress induction by H 2 O. control or celatrol-treated cells. Unit has several areas of damaged plastic. TGFα release was calculated as described in the Results. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. Microplate Readers. The Spectra Catalina 340 Automated Connect 12Volt or 24Volt Catalina 340C produces 14 gallons (53 liters) of water per hour, making it the perfect watermaker for the midsize. The SpectraMax® i Series Multi-Mode Mode Microplate Readers offer the ideal solution for any . 5 mL sample or standard solution was well mixed with 0. 2. This light offers spectrum variability, wireless control, and photosensors to automatically balance output with natural light. Screening for Antigen-Specific IgG Producing Clones by Capture ELISA. Continuous kinetic monitoring of enzyme activity was performed on SPECTRAmax 340 or Flexstation 2-384 microplate reader (Molecular Devices, Sunnyvale, CA, USA) and controlled by the Softmax software. Molecular Devices SpectraMax Gemini XS Microplate Reader. , Sunnyvale, CA). Absorbance at 490 nm was determined using a SPECTRAmax 340 (Molecular Devices Corporation). To correct for inter. Awareness Technology Inc. S. Both readers feature an 8-channel read head for fast reads and. Bertin Precellys Evolution Touch Homogenizer. View the LabX ads below to find Molecular Devices SpectraMAX plate readers for sale and. (1993). When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. LDH release was measured with a commercial kit following the manufacturer’s instructions (LDH Cytotoxicity Detection Kit, TaKaRa). gp70 blocking. 5. After 72 h, 20 μl of 5 mg/ml MTT solution was added to each well and plates were incubated for 3 h at 37°C. Rapid, accurate DNA, RNA, and protein quantitation in a one-touch, full-spectrum micro-volume absorbance reader. 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-ModeOpening the Drawer Manually on page The SpectraMax ABS can accommodate standard 96-well plates. 306 plate air cal. below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. The concentration of NO in the cells was determined by measuring the amount of nitrite, a relatively stable oxidation product of. Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. The absorbance values were measured at 450 nm using a SpectraMax® 340 plate reader (Molecular Devices, Sunnyvale, CA, USA). The reaction mixture consists of 100 mM potassium phosphate buffer (pH 7. 340 nm / 80 nm. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideHigh-throughput assays for promiscuous inhibitors Brian Y Feng1,3, Anang Shelat1,3, Thompson N Doman2,RKipGuy1 & Brian K Shoichet1 High-throughput screening (HTS) searches large libraries of chemical compounds. Absorbance plate reader, temperature control, robotics-compatible, 384 well plates, 340-850 nm. Customer Transformation Hub. 2 g/L solution; dilute 1:10 in HBSS. 6. 313 reference gain check fail. Manager: Yinghua Chen, PhD Contact Email: yinghua. Methods: We investigated growth inhibition by varying concentrations of AMB in seven isolates of C. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. According to LDH assay, a 60 μL of reaction mixture containing catalyst (diaphorase/NAD+), dye solution of iodotetrazolium chloride and sodium lactate was. plates can be used in the SPECTRAmax PLUS 384. Donor: 616/10 nm. The light features a slim profile to prevent. Microplate Readers. SoftMax Pro. The ratio of red to green fluorescence intensity was calculated. using SpectraMax i3 (Molecular devices, San Jose, USA). The optical density of each well was determined immediately using a microplate reader (SpectraMax-340, Molecular Devices) set to 450 nm. 2. used. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. 6. It includes both a lamp and a new lamp holder. Optical density was measured using a SpectraMAX 340 (Molecular Devices, Sunnyvale, CA). Afterwards, absorbance at 595 nm was measured with spectrophotometer (SpectraMax 340, Molecular Devices, USA). Manual: SoftMaxPro User Guide Manufacturer: Molecular Devices Location: SOM-E536. Cell culture 3T3 fibroblasts and RAW 264. 00 USD. The plates were read using an ELISA microplate reader (Molecular Devices SPECTRAMAX 340 PC 384, Sunnyvale, California, USA) at 450 nm. 6, 2 mmol/l DTT, 10 mmol/l MnCl 2 and 10 mmol/l pNPP; the PP2Cα enzyme concentration was 400nmol/l. SpectraMax Multi-Mode Microplate Readers. SpectraMax Injector Cartridge with SmartInject™ Technology. Proteolytic activity was determined using the synthetic chromogenic substrate Nα-benzoyl-DL-arginine p-nitroanilide (DL-BApNA), in 96-well plates, according to Ponce-Soto et al. Lactate dehydrogenase (LDH) release assay Cytotoxicity was determined by measuring the release of LDH. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. The extinction coefficient at 280 nm used for determining the concentration of Rsp3690 was calculated to be 28,795 M –1 cm –1. (7. Triton X-100 (see Note 2). TGFα release was calculated as described in the Results. The cell growth was determined by measuring the optical density at 600 nm using a spectrophotometer (SpectraMax 340, Molecular Devices, USA). The absorbance is determined at 620 nm on a SpectraMax 340 PC 384. Manager: Yinghua Chen, PhD Contact Email: yinghua. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Title: SPECTRAmax 340 Microplate Reader manual Author: Research Facilities Keywords: spectramax, 340, microplate reader, manual, Created Date: 12/13/2005 9:07:53 AMSpectraMax Multi-Mode Microplate Readers. 001/min at 425 nm. Fig. Bioz Stars score: 86/100, based on 1 PubMed citations. Each experiment was performed in duplicate. 6. Nitrocefin hydrolysis was monitored at 482 nm on a SpectraMax 340 UV-visible plate reader for high-throughput assays and an HP8453 UV-visible spectrophotometer for the low-throughput assays. When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Following HRP-conjugated secondary antibodies, goat anti-rat IgG (Kirkegaard & Perry. Binding affinity for denosumab. Service Contracts, Preventative Maintenance and Performance. Examples: “Press carefully to engage,” “Do not press down. The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance measurement in the visible range. Subsequently, the mixture was heated at 100 °C for 10. Absorbance was read at 405 nm on a microplate reader (SPECTRAmax 340 PC). ): 8. 340/70 nm. Fully tested with Warranty. Apoptosis was also measured by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling (TUNEL) assay using the TACS Blue label kit (4811-30-K, R&D Systems). Alves, Amelia K. Analytical Data Regression and Acceptance Limits The conversion of the optical density units for the test samples, VSs, and QCs to concentrations was performed using the Watson LIMS software (version. 316 grating motor driver faulty. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. Figure 1. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. Please see photos. The absorbance was measured at 340 nm for 2 min at a 15 s time interval. Readings were made in a microplate spectrophotometer (SpectraMAX 340, Molecular Devices, Sunnyvale, California, USA) with wavelength set to 450 nm and correction set. 7. After 24 h, 48 h, 72 h or 96 h, cell viability was measured by CellTiter 96® AQueous One Solution cell proliferation assay (Promega, Madison, WI, USA). Other technologies such as imaging. According to LDH assay, a 60 μL of reaction mixture containing catalyst (diaphorase/NAD+), dye solution of iodotetrazolium chloride and sodium lactate was. 0 0 The indicated microplates were scanned form 200 nm to 800 nm. RESEARCH ARTICLE Effects of early-life conditions on innate immune function in adult zebra finches Merijn M. SpectraMax 340 (Serial number starting with M) SpectraMax 340PC (Serial number starting with L) SpectraMax Plus (Serial number starting with P) Tmax Also refer to the Obsolete Microplate Reader Models article for additional information. Or scan up to 384 wells at any time to determine the best assay conditions. Recalcification (clotting) time was determined by monitoring absorbance at 650 nm at 10-sec intervals in a SpectraMax 340 microtiter plate reader, with onset time (the time to a linear increase in the OD, which reflects the maximal rate of formation of insoluble fibrin) set at an OD of 0. Finally, after another 45 min of decolorization, 100 µL of the solution was transferred to a new well and the amount of crystal-violet stain in the decolorizing solution was measured with a microtiter plate reader (SpectraMAX 340 Adjustable Microplate Reader; Molecular Devices Ltd. The SPECTRAmax 340PC can mea-The extracted stain was then transferred to a 96-well plate, and the absorbance at 562 nm was measured using a SpectraMax 340 plate reader/spectrophotometer(Molecular Devices Corp. 1: SpectraMax Plus 384 components. The microtiter plate was then incubated in the dark for 2 h at 37°C. Not all manufacturers' microplates are the same with regard to design, materials, orIn a typical 100-μL assay with mixtures containing 50 mM MOPS, pH 6. Then, the absorbance value at 450 nm was determined using a microplate reader (SpectraMAX 340; Molecular Devices, Sunnyvale, CA, United States). edu Contact Phone: 216-368-8962. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. SpectraMax 340PC384 reads 96-well plates and 384-well plates and strip-well plates. Detection limits. FTIR Spectrometer: Shimadzu IRSpirit (2018)The data were acquired with a Molecular Devices SpectraMax 340 Microplate Reader (Sunnyvale, CA, USA). The SpectraMax i3x reader provides the flexibility to add novel detection capabilities without the need for service engineers or costly downtime—it grows with you as your research areas expand. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. 024). Permeability of the monolayer was verified by adding 1% Evans Blue dye (Thermo Fisher Scientific, Inc. Products : SpectraMax Plus 384 reader,. Studies of the regulation of AoSMC migration by Angpt2 were preformed using a wound healing assay. 0. Incubate 30 min at room temperature, and then read microplates at 405 nm using SpectraMax 340 PC plate reader (Molecular Devices) (see Note 8). Lactate Dehydrogenase (LDH) Release. Data System. The inhibition of ALDH activity by compounds and the IC50 curves were determined by measuring the formation of NAD(P)H spectrophotometrically at 340 nm (molar extinction coefficient of 6200 M −1 cm −1) on a Beckman DU-640 as well as a Spectramax 340 PC spectrophotometer (GMI, Ramsey, NJ, USA) using a purified. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideMolecular Devices SpectraMax Gemini XS Microplate Fluorometer $2,800. The optical system of the SpectraMax 340PC384 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for. CD154 detection in equine PBMC by an anti-human CD154 antibody (clone 5C8) was confirmed by Western blots and then applied for flow cytometry. #W50-640) SpectraMax i3 or i3x Multi-Mode Microplate Reader (Molecular Devices; other SpectraMax microplate readers with fluorescence detection are also suitable) SoftMax Pro Software (Molecular Devices) Methods VersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. Subsequent replacements require only the lamp (N-34-LB) be replaced. A 340 μL of 50 mM borate buffer (pH 9), 240 μL methanol, 10 μL standard solution or sample, and 20 μL of 0. , Sunnyvale, CA). 0 3. . Emission. Stat Fax 4700 Microstrip Reader. The drawer will open and it may close, if is doesn't, close it using the DRAWER button found on the Spectramax control panel (pictured in purple on. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. On LabX buyers can find a variety of SpectraMAX models for sale: 190, 384, 340 and the SpectraMAX M series. Although it has been proven to be effective in various neurologic disorders, the mechanism of the treatment of traumatic brain injury (TBI) is not fully understood. SpectraMax Microplate Readers. Unit powers. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. . Excitation 1 340 nm Emission 1 520 nm Cut off for emission 1 495 nm Excitation 2 340 nm Emission 2 620 nm Cut off for emission 2 570 nm Sensitivity Readings 50 to 100 PMT Optimal Column Wavelenght Column Priority . Page: 1/6. After washing, the dye was eluted with 3% HCl, and the absorbance measured with a SpectraMax 340 microtiter plates reader at 665 nm. Molecular Devices SPECTRAmax. The contents of the. For use with Spectramax 340 devices which have an 8-pin mini DIN serial socket. 5. , Fremont, CA, USA). The. Photometric Range: 0. IC 50 values were determined by mathematical curve-fitting (SoftMaxPro Molecular Devices) and were defined as the drug concentration leading to 50% inhibition of net cell mass. Although not statistically significant, pain was reduced by 2 NRS steps or more during supplemental treatment with naloxone in 36% of subjects when. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Labsystems Luminoskan RT Microplate Reader. 8. Cell proliferation was measured using the MTT method. The activity was measured in a SpectraMAX 340 plate reader at 37°C and 410 nmol/l for 120 sec. 4) was followed according to Vanacker et al. This is equivalent to having 511 built-in filters,Results: A total of 64% of the subjects reported improved quality of sleep during treatment with naloxone at a dose of 40 ng per 24 hours as compared with 9% with sham treatment (P=0. All assays were done in triplicate with readings at 60-s intervals using a SpectraMax 340 multiwell plate reader (Molecular Devices, San Jose, CA, United States). The OD for each serum is adjusted by subtracting either the OD for spontaneous mediator release or antigen-specific mediator release. 8 (w) x 15 (d) Performance Specifications Wavelength Range: 340 – 850 nm Wavelength Selection: Monochromator, tunable 1. Absorbance was recorded at 540 nm using SpectraMAX 340 reader. the SpectraMax iD3 is a fantastic plate reader for a wide range of assays. ABS reader ranges from 340-850 nm in wavelength and the ABS Plus reader ranges from 190-1000 nm, which is equivalent to having 510 or 810 free filters, respectively. 30. 5 mL of 1 M acetate buffer (pH 4. Time-resolved fluorescence was measured on the SpectraMax i3x and SpectraMax iD5 readers using preconfigured protocols in SoftMax® Pro Software (see Tables 2 and 3 for instrument-specific settings). ” SpectraMax® Quant™ dsDNA Assay Kits The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. 0 Micromoles per Joule, ensuring the highest light output using the least amount of electricity. Bacterial growth was measured after every 45 min by recording the absorbance at 595 nm using a SpectraMax 340 spectrophotometer (Molecular Devices, Sunnyvale, CA, USA). SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. All variables were tested in three independent cultures for each experiment, which was repeated twice (n = 6). 20. Related Products: SpectraMax 340 Absorbance Microplate Reader, Molecular Devices SpectraMax 384 Plus UV/Vis Microplate Reader, Molecular DevicesGETTING THE MOST FROM YOUR MOLECULAR DEVICES MICROPLATE SYSTEMS applications guide to microplate systems applications guide to microplate systems450 nm using a SpectraMax 340 microplate reader (Molecular Devices, Sunnyvale, CA, USA). 2 Chemical Reagents 1. After adding the stop solution, the absorbance at 450 nm with a wavelength correction at 540 nm was measured with a SpectraMax 340 plate reader (Molecular Devices, San Jose, CA, USA). Temperature Range: Ambient + 4 °C up to 45 °C. SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2 e. Absorbance was measured at 450 nm with ELISA Reader (SpectraMax 340, Molecular Device Co. Spectramax 340pc User Manual, Hudson River Saranac Pellet Stove Manual, Alesis Midiverb 1 Manual, User Manual For Samsung Galaxy Ace Duos S6802, Di Style Manual, R150f Transmission Repair Manual, Eos5d Mark 2 Manual. Data are expressed as percent of maximum binding for each protein. Our absorbance plate readers feature our PathCheck Sensor technology and. Although all samples initially were analyzed at the same volume, the volume of sample. 05 ng/mL; progesterone: 2. The reaction mixture was incubated for 5 min. Total Cell Count in BALF Cells collected from the lavage fluid were suspended in 1 mL of F-12 media supplemented with 10% FBS. Availablility: By Availability Molecular Devices SpectraMAX 340 Microplate Reader. This is equivalent to having 511 built-in filters,Colour change was measured in a SpectraMAX ® 340 spectrophotometer. Briefly, 50 µl-samples were incubated with equal volumes of Griess reagent (1% sulphanilamide, 0. Values are expressed as the mean s ± SD of 3 separate experiments, each performed in triplicate. Afterwards, absorbance at 595 nm was measured with spectrophotometer (SpectraMax 340, Molecular Devices, USA). Fios Router Setup Manual, Losi Xx4 We Manual, Statics Hibbeler Solutions Manual 12th Edition, Nokia Hs 23 Manual, Spectramax 340 Manual, Nolan N102 User Manual, Ship Security Officer Manual yunqian 4. Each experiment was performed in duplicate and repeated at. Absorbance values were measured using a plate reader (Molecular Devices; SpectraMax 340) at λ = 405 nm. SpectraMax190,340PC384,Plus384,VersaMaxMicroplateSpectrophotometersUserGuide 4 0112-0126D SafetyInformation. The optical density of each sample was analyzed at 450 nm with a reference reading at 630 nm using a SpectraMax 340 absorbance plate reader (Molecular Devices, Union City, CA). Cell Assay Cells (5 × 10 5 per well) are plated in triplicate in 96-well plates in 0. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. Absorbance was then measured at 540 nm (isosbestic point of cytochrome c) and 550 nm (SpectraMax 340; Molecular Devices, Sunnyvale, CA, USA). Availablility: By Availability Molecular Devices SpectraMAX 340 Microplate Reader. Number of flashes. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deep UV spectra must be used. The absorbance is determined at 620 nm on a SpectraMax 340 PC 384. Alkaline phosphatase (ALP) activity. SpectraMax Multi-Mode Microplate Readers. 6) and 0. Background: Ketamine can act as a multifunctional neuroprotective agent by inhibiting oxidative stress, cellular dysfunction, and apoptosis. The absorbance at 450 nm was determined using a microplate reader (spectraMAX 340). Softmax PRO v5. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. Not all manufacturers' microplates are the same with regard to design, materials, orSpectraMaxABS Plus,SpectraMaxM2,SpectraMaxM2e,SpectraMax M3,SpectraMaxM4,SpectraMaxM5,SpectraMaxM5e,SpectraMaxPlus 384 0200-7200 Multi-Mode ValidationPlate. When you do reads at wavelengths below 340 nm, you must use special UV-transparent, disposable or quartz plates to allow transmission of the deep UV spectra. automated SpectraMAX 340 (Molecular Devices, Sunnyvale, CA, USA) microtiter plate reader. SpectraMax Multi-Mode Microplate Readers. Dissolve 13. 30. When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Description Control Panel Cuvette Chamber Back Panel Microplate Drawer The Control Panel TEMP on/off TEMP Figure 1. 00. SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2 e. Software (SoftMax Pro ® software or comparable plate reader software). Absorbance was measured at 490/650 nm by using a SpectraMax 340 microplate spectrophotometer® (Molecular Devices, Sunnyvale, CA, U. SpectraMax Multi-Mode Microplate Readers. Irene Tieleman, Ido PenandSimon VerhulstSubsequently, the oxidative burst was initiated by the addition of PMA (final concentration 100 nM) and the cells incubated for 120 min at 37°C. The control panel consists of a 2-x-20-character LCD and eleven pressure-sensitive membrane keys that can be used to control some functions of the instrument. 30. Filterless readers provide consistent results with steady temperature regulation. #W50-640) SpectraMax i3 or i3x Multi-Mode Microplate Reader (Molecular Devices; other SpectraMax microplate readers with fluorescence detection are also suitable) SoftMax Pro Software (Molecular Devices). The SpectraMax™ GEMINI EM Dual-Scanning Microplate Spectrofluorome-ter can perform a variety of fluorescent applications as well as some lumines-cent assays. The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid- pathways and protein activation and expression in one system. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating. Briefly, cells were seeded into a. 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. Cet appareil ISM est confomre à la norme NMB-001 du Canada. Fibrin clots were formed as described above with some modifications 46. Quick View. Molecular Devices Spectramax 340 Absorbance Plate Reader (1997) Molecular Devices SpectraMax i3x Multi-Mode Microplate Reader (2016) Vernier UV-VIS Spectrophotometer (5) Vernier VIS Spectrophotometer (30) Infrared Spectroscopy. Isolation of RNA and RT-PCR. When you do reads at wavelengths below 340 nm, you must use special UV-transparent, disposable or quartz plates to allow transmission of the deep UV spectra. Also for: Spectramax 190, Spectramax 340pc384, Versamax. StakMax Microplate Handling Stacker. After adding sample, the mixture was incubated for 30 min at 37°C, with one unit of enzymatic activity being defined as an increase in absorbance of 0. Although it has been proven to be effective in various neurologic disorders, the mechanism of the treatment of traumatic brain injury (TBI) is not fully understood. Features showcased by this model include temperature control, a robotics-compatible interface and Data Analysis Software. These isotypes cause antigen cross-linking by multimeric binding and self. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. The screening was initiated by adding 10 μL p-nitrophenyl phosphate (pNPP), and the catalysis of pNPP (1 mM) was continuously monitored on SpectraMax 340 microplate reader (Molecular Devices, Sunnyvale, CA, USA) at 405 nm for 20 min at 37 °C. Key benefits • User-upgradeable application modules including cellular imaging SpectraTest ABS1 Absorbance Validation Plate. , Oxon, UK). BV-2 cells were treated with various concentrations of BT and/or LPS for 24 hFollowing incubation, absorbance was immediately measured at 405 nm with a SpectraMax 340 spectrophotometer (Molecular Devices, LLC. The growth. Created Date: 20051213090753Zmeasured using a SpectraMax 340 multiwell plate. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Molecular Devices ThermoMax is a high-efficiency, temperature-regulated, 96-well microplate reader. On-site Repair Service available nationwide for Molecular Devices Spectramax 340 Plate Reader's. Absorbance values were read at 570 nm on an automated SpectraMAX 340 (Molecular Devices, Sunnyvale, CA, USA). 0 OD. They are for reference only. Acceptor: 665/10 nm. Absorbance was then measured at 540 nm (isosbestic point of cytochrome c) and 550 nm (SpectraMax 340; Molecular Devices, Sunnyvale, CA, USA). Manual: SoftMaxPro User Guide Manufacturer: Molecular Devices Location: SOM-E536. 5 ng/ml; estradiol: 10 pg/ml. Plates were read at a wavelength of 405 nm with a reference wavelength of 620 nm (Spectramax 340 PC). 6, 2 mmol/l DTT, 10 mmol/l MnCl 2 and 10 mmol/l pNPP; the PP2Cα enzyme concentration was 400nmol/l. The Gemini EM Reader with dual monochromators allows researchers to try new and novel dyes without having toThe absorbance was determined to be 450 nm using an ELISA reader (SpectraMax® 340, Molecular Device Co. Each experiment was performed in duplicate. The activity was measured in a SpectraMAX 340 plate reader at 37°C and 410 nmol/l for 120 sec. One plate carrier adapter is provided with the instrument. Optical density was measured at 570 nm using a microplate reader (SpectraMAX 340, Molecular Devices Co. chen@case. Filipin StainingSpectraMax® 340PC384 microplate reader provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro data analysis software. Narrow bandwidths deliver increased measurement accuracy and linearity over a wide range of assays. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. 7 macrophages were obtained from ATCC (Manassas, VA). ZERO BIAS - scores, article reviews, protocol conditions and moreAbsorbance was measured at 450 nm with ELISA Reader (SpectraMax 340, Molecular Device Co. 2. SpectraMax® Microplate Readers and SoftMax® Pro Software. TDP-43 stress granular cells were treated with 70% ice-cold. The absorbance at 540 nm was determined in a spectrophotometer (SPECTRAmax 340, Molecular Devices). 0% and ± 0. Supplier: Molecular Devices. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. For more sample throughput, the system canThe optical density (OD) at the wavelength of 450 nm was measured with a spectrometer (SpectraMax 340, Sunnyvale, CA, USA).